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KMID : 0382619840040010307
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Hanyang Journal of Medicine
1984 Volume.4 No. 1 p.307 ~ p.319
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A Study of Exoenzymes of Pseudomonas aeruginosa
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崔炳籌/Choi, Byung Joo
趙陽子/徐仁銖/Cho, Yang Ja/Suh, Inn Soo
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Abstract
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Protease and elaetase production and their mouse lethal activity of Pseudomonas aeruginosa were studied with sixteen clinical isolates by comparing animal passed strains as in vivo with subcultivated strains on medium containing either substrate of casein or elastin as in vitro.
The results obtained were as follows.
1. On proteas and elastase production of strains passed serially in mice,6 out of 7 original strains of both enzymes non-producing were converted into both positive, 6 out of 8 original strains of protease non-producing remajed negative and 2 were converted into positive, and 3 strains showed more potent positive response as the number of passage increased.
2. On lecithinase production of strains passed serially in mice, 7 out of 16 original strains were converted into positive and persistent negative strains were 5. On hemolysin production of strains passed serially in mice, 4 out of 16 original strains were converted into positive and 2 strains showed negative persistently.
3. Comparing virulence between the original and the mouse passed strains, the latters were the more potent than the formers without paying regard to extracellular enzyme production including protease and elastase.
4. On protease and elastase production of strains subcultivated on medium comtaining either of the substrastes, 4 out of 6 original strains of both enzymes non-producing were converted into both positive and showed more potent pesponse as the number of subcultivation increased and 4 out of 8 original strains of protease non-producing were converted into positive of protease production at the first to third subcultivation and showed ond to two positive within 2 days and three positive after 3 days of incubation.
5. On mouse lethal activity of protease and elastase producing and non-producing strains, the LD?? was 10?? to 10?? Cells per mouse and there were no significant differences between the challenging routes of intravenous and intraperitoneal.
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KEYWORD
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